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Vero Cell Rabies Vaccine, supplied by Sanofi, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vero cells  (Korean Cell Line Bank)
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Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus <t>production.</t> <t>HEK293FT</t> cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect <t>Vero</t> cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).
Vero Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vero 76 cells  (ATCC)
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Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus <t>production.</t> <t>HEK293FT</t> cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect <t>Vero</t> cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).
Vero 76 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vero e6 cells  (ATCC)
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Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus <t>production.</t> <t>HEK293FT</t> cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect <t>Vero</t> cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).
Vero E6 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vero cell  (ATCC)
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Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus <t>production.</t> <t>HEK293FT</t> cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect <t>Vero</t> cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).
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vero african green monkey kidney cells  (ATCC)
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ATCC vero african green monkey kidney cells
Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus <t>production.</t> <t>HEK293FT</t> cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect <t>Vero</t> cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).
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vero cell host cell proteins elisa kit  (Cygnus Technologies)
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Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus <t>production.</t> <t>HEK293FT</t> cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect <t>Vero</t> cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).
Vero Cell Host Cell Proteins Elisa Kit, supplied by Cygnus Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cervical carcinoma cells  (ATCC)
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Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus <t>production.</t> <t>HEK293FT</t> cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect <t>Vero</t> cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).
Human Cervical Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vero cell monolayer cytotoxicity assays  (ATCC)
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ATCC vero cell monolayer cytotoxicity assays
Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus <t>production.</t> <t>HEK293FT</t> cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect <t>Vero</t> cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).
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cell line  (ATCC)
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ATCC cell line
Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus <t>production.</t> <t>HEK293FT</t> cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect <t>Vero</t> cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).
Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus production. HEK293FT cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect Vero cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).

Journal: Frontiers in Immunology

Article Title: Development of Nipah virus mRNA vaccine for pandemic preparedness

doi: 10.3389/fimmu.2026.1843559

Figure Lengend Snippet: Production of NiV pseudovirus using the established pseudovirus system. (A) Schematic workflow of lentivirus-based and VSV-based Nipah virus pseudovirus production. (B) Confirmation of protein expression during lentivirus-based Nipah pseudovirus production. HEK293FT cells were transfected with plasmids required for lentivirus-based Nipah pseudovirus production, and protein expression was examined at 24 h and 48 h post-transfection using bright-field microscopy (left) and fluorescence microscopy (right). (C) Observation of cellular changes during VSV-based Nipah pseudovirus production. HEK293FT cells expressing NiV-F and NiV-G (left) and cells without expression of any viral proteins (right) are shown. Cells were infected with Δ G-VSV-Luc, and cellular morphology was examined 24 h post-infection. (D) Evaluation of infectivity of lentivirus-based Nipah pseudovirus. The produced lentivirus-based Nipah pseudovirus was used to infect Vero cells, and infectivity was assessed by measuring RLU using a luciferase assay at 24 h post-infection. Experiments were performed twice, and statistical analysis was conducted using an unpaired t-test (P ≥ 0.05). (E) Evaluation of infectivity of VSV-based Nipah pseudovirus. Vero cells were infected with the produced VSV-based Nipah pseudovirus or Bald VSV, and infectivity was assessed by measuring RLU values. Experiments were performed in triplicate, and statistical analysis was conducted using an unpaired t-test (*P< 0.05).

Article Snippet: HEK293FT and Vero cells (Korean Cell Line Bank, Seoul, Korea) were cultured in DMEM (Cytiva, Washington, USA) and RPMI (Cytiva, Washington, USA) containing 10% fetal bovine serum (FBS, Cytiva, Washington, USA) and 1% antibiotic–antimycotic (Gibco, Massachusetts, USA).

Techniques: Virus, Expressing, Transfection, Microscopy, Fluorescence, Infection, Produced, Luciferase